EePiEn – SI Steinernema indica Entomo Pathogenic Nematode for Biological Pest Control of Coffee Stem Borer, Stem / Shoot borers in Orchards, Plantation and Field Crops Unit of packaging Steinernema indica (Entomo Pathogenic Nematode) EePiEn - SI pack size: Bag in a box Contains: 5 million larvae (third stage) in inert carrying material Preparation of solution Put the contents of the package into a bucket containing 5 litres of water (15-20°C/59-68°F) Stir well and leave the solution to soak for 20-30 seconds Stir well again and pour the entire content into spray tank Fill up with the required amount of water Spray immediately after preparation Use the whole package at once, the distribution of nematodes in the pack is not homogenous! Target Larvae of Coffee Stem Borer, Stem / Shoot Borers in orchard trees / plantation crops Application rate     EePiEn-SI Rate M²/Unit Interval (days) Frequency Preventive - - - - Curative light 500000/m² 100 - 1x curative heavy 500000/m² 100 7  2-3x Application method Apply with a watering-can, Mix in irrigation system, Spray in knapsack or motorized sprayers To avoid blockage of nozzles, all filters should be removed Use a maximum pressure of 12 bar in Sprayer The spray nozzle opening should be at least ½ mm (500 micron) Spread the spraying solution evenly over the area to be treated Continuous mixing should take place to prevent nematodes in EePiEn - SI sinking to the bottom of the spray tank Soil application Apply @ 0.25-1 million EePiEn - SI per m² Quantity of spray solution depends on application method Water the crop before and after application Keep the soil moist for the first 2 weeks after application   Foliar application Use 0.25 million EePiEn - SI per m² dissolved in 0.125 lt spray solution (=1250 Lt/Ha) Add some non ionic wetting agent to improve efficacy Spray the crop evenly with the EePiEn - SI solution Repeat treatment weekly as long as necessary   Environmental conditions Humidity content of the soil must be high Soil temperature between 13-25°C/56-77°F   Storage and handling Storage after receipt: see package for expiry date Storage temperature: 2-6°C/36-42°F Store in dark cabinet Remove product from insulating outer for rapid cooling of content   Appearance Larvae (third stage): size 0.7-1.0 mm Other stages: develop inside host   Mode of action The nematodes in EePiEn - SI enter the larva of the Coffee Stem Borer / stem borers / shoot borers via mouth, anus or respiratory openings of pest and start to feed. This causes specific bacteria Xenorhabdus nematophila to emerge from the intestinal tract of the nematode. The bacteria spreads inside the insect and multiply very rapidly. The bacteria converts host tissue into products which can easily be taken up by the nematodes. The host dies within a few days.   Visual effect Infected insects develop yellow to (light) brown colour and become slimy. Therefore, they are difficult to observe in the soil.   Important! The introduction rates of EePiEn - SI should be adjusted to the mode of action of the product and the results that can be expected in the crop where the product is applied.